Publications

  1. Alexander MS, O’Leary BR, Moose D, Du J, Henry MD, Cullen JJ. A model for the detection of pancreatic ductal adenocarcinoma circulating tumor cells. J Biol Methods. 2018 Sep 5;5(3):e97. doi: 10.14440/jbm.2018.250. PMID: 31453247; PMCID: PMC6706145.
  2. Schoenfeld JD, Alexander MS, Waldron TJ, Sibenaller ZA, Spitz DR, Buettner GR, Allen BG, Cullen JJ. Pharmacological Ascorbate as a Means of Sensitizing Cancer Cells to Radio-Chemotherapy While Protecting Normal Tissue. Semin Radiat Oncol. 2019 Jan;29(1):25-32. doi: 10.1016/j.semradonc.2018.10.006. PMID: 30573181; PMCID: PMC6310038.
  3. Alexander MS, Cullen JJ. Treating pancreatic cancer: more antioxidants more problems? Expert Rev Gastroenterol Hepatol. 2018 Sep;12(9):849-851. doi: 10.1080/17474124.2018.1494572. Epub 2018 Jul 5. PMID: 29956580; PMCID: PMC6317716.
  4. O’Leary BR, Houwen FK, Johnson CL, Allen BG, Mezhir JJ, Berg DJ, Cullen JJ, Spitz DR. Pharmacological Ascorbate as an Adjuvant for Enhancing Radiation-Chemotherapy Responses in Gastric Adenocarcinoma. Radiat Res. 2018 May;189(5):456-465. doi: 10.1667/RR14978.1. Epub 2018 Mar 16. PMID: 29547353; PMCID: PMC6117840.
  5. Brandt KE, Falls KC, Schoenfeld JD, Rodman SN, Gu Z, Zhan F, Cullen JJ, Wagner BA, Buettner GR, Allen BG, Berg DJ, Spitz DR, Fath MA. Augmentation of intracellular iron using iron sucrose enhances the toxicity of pharmacological ascorbate in colon cancer cells. Redox Biol. 2018 Apr;14:82-87. doi: 10.1016/j.redox.2017.08.017. Epub 2017 Aug 26. Erratum in: Redox Biol. 2018 Jun 8;: PMID: 28886484; PMCID: PMC5591450.
  6. Alexander MS, Wilkes JG, Schroeder SS, Buettner GR, Gibson-Corley K, O’Leary BR, Spitz DR, Buatti JM, Berg DJ, Bodeker KL, Vollstedt S, Brown HA, Allen BL, Cullen JJ. Pharmacological ascorbate reduces radiation-induced normal tissue toxicity and enhances tumor radiosensitization in pancreatic cancer. Cancer Research, 78:6838-6851, 2018 DOI:10.1158/0008-5472.CAN-18-1680. NIHMS 1511241.
  7. Alexander MS, O’Leary BR, Wilkes JG, Gibson AR, Wagner BA, Du J, Sarsour E, Hwang RF, Buettner GR, Cullen JJ. Enhanced Pharmacological Ascorbate Oxidation Radiosensitizes Pancreatic Cancer. Radiation Research 191:43-51, 2019. NIHMS 1008361.
  8. Cullen JJ. The dual effect of pharmacological ascorbate on radiation: the best of both worlds. Oncotarget, 2018 Nov 30;9(94):36648-36649. doi: 10.18632/oncotarget.26396.
  9. Cullen JJ, Alexander MS. Pharmacological Ascorbate as a Radiosensitizer. In Part II, Mechanism of Action for Vitamin C in Cancer. Chapter 5, IN: Cancer and Vitamin C. Edited by Qi Chen and Jeanne Drisko. CRC press. 2019.
  10. Petronek MS, Wagner BA, Hollenbeck NJ, Caster JM, Spitz DR, Cullen JJ, Buettner GR, Allen BG. Assessment of the stability of supra-physiological ascorbate in human blood: Appropriate handling of samples from clinical trials for measurements of pharmacological ascorbate. Radiation Research, 2019 Jun;191(6):491-496. doi: 10.1667/RR15328.1. 2019.
  11. Buranasudja V, Doskey CM, Gibson AR, Wagner BA, Du J, Gordon DJ, Koppenhafer SL, Cullen JJ, Buettner GR. Pharmacological Ascorbate Primes Pancreatic Cancer Cells for Death by Rewiring Cellular Energetics and Inducing DNA Damage. Molecular Cancer Research, 17:2102-2114, 2019 10.1158/1541-7786.MCR-19-0381.
  12. Allen B, Bodeker K, Smith M, Monga V, Sandhu S, Hohl R, Carlisle T, Brown H, Hollenbeck N, Vollstedt S, Greenlee J, Howard M, Seyedin S, Caster J, Jones K, Cullen J, Berg D, Wagner B, Buettner G, TenNapel M, Smith B, Spitz D, Buatti J. First-in-human phase 1 clinical trial of pharmacological ascorbate combined with radiation and temozolomide for newly diagnosed glioblastoma. Clinical Cancer Research 25:6591-6597, 2019. DOI: 10.1158/1078-0432.
  13. Klener P, Alexander MS, Cullen JJ, Stejskal V, Slíva J, Kotlářová L, Kostiuk P, Procházka Z, Kučerová M. The benefits of ascorbate to protect healthy cells in the prevention and treatment of oncological diseases. Journal of Applied Biomedicine. P. 1-7, 2020DOI: 10.32725/jab.2020.003
  14. Gibson AR, O’Leary BR, Du J, Sarsour EH, Kalen AL, Wagner BA, Stolwijk JM, Falls-Hubert KC, Alexander MS, Carroll RS, Spitz DR, Buettner GR, Goswami PC, Cullen JJ. Dual oxidase-induced sustained generation of hydrogen peroxide contributes to pharmacological ascorbate-induced toxicity. Cancer Research, 80:1401-1413, 2020. doi: 10.1158/0008-5472.CAN-19-3094.
  15. Sarsour EH, Son JM, Kalen AL, Xio W, Du J, Alexander MS, O’Leary BR, Cullen JJ, Goswami PC. Arachidonate 12-Lipoxygenase and 12-Hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer. Journal of Biological Chemistry. 295:6946-6957, 2020. PMID: 32265301 PMCID: PMC7242692
  16. Carroll RS, Buettner GR, Cullen JJ. Pharmacological Ascorbate and Use in Pancreatic Cancer. IN: Cancer: Oxidative Stress and Dietary Antioxidants. Edited by Victor Preedy.
  17. O’Leary BR, Alexander MS, Du J, Moose DL, Henry MD, Cullen JJ. Pharmacological ascorbate inhibits pancreatic cancer metastases via a peroxide-mediated mechanism. Scientific Reports, (2020) 10:17649. doi.org/10.1038/s41598-020-74806-2. PMID: 33077776 PMCID: PMC7572461
  18. Cushing CM, Petronek MS, Bodeker KL, Vollstedt S, Brown HA, Opat E, Hollenbeck NJ, Shanks T, Berg DJ, Smith BJ, Smith MC, Monga V, Furqan M, Howard MA, Greenlee JD, Mapuskar KA, St-Aubin J, Flynn RT, Cullen JJ, Buettner GR, Spitz DR, Buatti JM, Allen BG, Magnotta VA. Magnetic Resonance Imaging (MRI) of Pharmacological Ascorbate-Induced Iron Redox State as a Biomarker in Subjects Undergoing Radio-Chemotherapy. Redox Biology, in press https://doi.org/10.1016/j.redox.2020.101804.
  19. Callaghan CM, Hasibuzzaman MM, Rodman SN, Goetz JE, Mapuskar KA, Petronek MS, Steinbach EJ, Miller BJ, Pulliam CF, Coleman MC, Monga VV, Milhem MM, Spitz DR, Allen BG. Neoadjuvant Radiotherapy-Related Wound Morbidity in Soft Tissue Sarcoma: Perspectives for Radioprotective Agents. Cancers (Basel). 2020 Aug 12;12(8):2258. doi: 10.3390/cancers12082258. PMID: 32806601; PMCID: PMC7465163.
  20. Buettner GR, Spitz DR, Limoli CL. Response to Ling et al. regarding “An integrated physico-chemical approach for explaining the differential impact of FLASH versus conventional dose rate irradiation on cancer and normal tissue responses”. Radiother Oncol. 2020 Jun;147:241-242. doi: 10.1016/j.radonc.2020.03.001. Epub 2020 Mar 25. PMID: 32222332; PMCID: PMC8757288.
  21. Gibson AR, O’Leary BR, Du J, Sarsour EH, Kalen AL, Wagner BA, Stolwijk JM, Falls-Hubert KC, Alexander MS, Carroll RS, Spitz DR, Buettner GR, Goswami PC, Cullen JJ. Dual Oxidase-Induced Sustained Generation of Hydrogen Peroxide Contributes to Pharmacologic Ascorbate-Induced Cytotoxicity. Cancer Res. 2020 Apr 1;80(7):1401-1413. doi: 10.1158/0008-5472.CAN-19-3094. Epub 2020 Feb 10. PMID: 32041838; PMCID: PMC7127976.
  22. Kalen AL, Wagner BA, Sarsour EH, Kumar MG, Reedy JL, Buettner GR, Barua NC, Goswami PC. Hydrogen Peroxide Mediates Artemisinin-Derived C-16 Carba-Dimer-Induced Toxicity of Human Cancer Cells. Antioxidants (Basel). 2020 Jan 26;9(2):108. doi: 10.3390/antiox9020108. PMID: 31991904; PMCID: PMC7070254.
  23. Stolwijk JM, Falls-Hubert KC, Searby CC, Wagner BA, Buettner GR. Simultaneous detection of the enzyme activities of GPx1 and GPx4 guide optimization of selenium in cell biological experiments. Redox Biol. 2020 May;32:101518. doi: 10.1016/j.redox.2020.101518. Epub 2020 Mar 29. PMID: 32278283; PMCID: PMC7150527.
  24. Erudaitius DT, Buettner GR, Rodgers VGJ. The latency of peroxisomal catalase in terms of effectiveness factor for pancreatic and glioblastoma cancer cell lines in the presence of high concentrations of H2O2: Implications for the use of pharmacological ascorbate in cancer therapy. Free Radic Biol Med. 2020 Aug 20;156:20-25. doi: 10.1016/j.freeradbiomed.2020.05.023. Epub 2020 Jun 6. PMID: 32522584; PMCID: PMC7434663.
  25. Brandt KE, Falls KC, Schoenfeld JD, Rodman SN 3rd, Gu Z, Zhan F, Cullen JJ, Wagner BA, Buettner GR, Allen BG, Berg DJ, Spitz DR, Fath MA. Corrigendum to: Augmentation of intracellular iron using iron sucrose enhances the toxicity of pharmacological ascorbate in colon cancer cells [Redox Biol. (2018) 82-87]. Redox Biol. 2020 Jan;28:100935. doi: 10.1016/j.redox.2018.06.001. Epub 2018 Jun 8. Erratum for: Redox Biol. 2018 Apr;14:82-87. PMID: 29891355; PMCID: PMC6920117.
  26. Falls-Hubert KC, Butler AL, Gui K, Anderson M, Li M, Stolwijk JM, Rodman SN 3rd, Solst SR, Tomanek-Chalkley A, Searby CC, Sheffield VC, Sandfort V, Schmidt H, McCormick ML, Wels BR, Allen BG, Buettner GR, Schultz MK, Spitz DR. Disulfiram causes selective hypoxic cancer cell toxicity and radio-chemo-sensitization via redox cycling of copper. Free Radic Biol Med. 2020 Apr;150:1-11. doi: 10.1016/j.freeradbiomed.2020.01.186. Epub 2020 Feb 4. PMID: 32032663; PMCID: PMC7299833.
  27. Kloepping KC, Kraus AS, Hedlund DK, Gnade CM, Wagner BA, McCormick ML, Fath MA, Seol D, Lim TH, Buettner GR, Goswami PC, Pigge FC, Spitz DR, Schultz MK. Triphenylphosphonium derivatives disrupt metabolism and inhibit melanoma growth in vivo when delivered via a thermosensitive hydrogel. PLoS One. 2020 Dec 30;15(12):e0244540. doi: 10.1371/journal.pone.0244540. PMID: 33378390; PMCID: PMC7773266.
  28. Stolwijk JM, Garje R, Sieren JC, Buettner GR, Zakharia Y. Understanding the Redox Biology of Selenium in the Search of Targeted Cancer Therapies. Antioxidants (Basel). 2020 May 13;9(5):420. doi: 10.3390/antiox9050420. PMID: 32414091; PMCID: PMC7278812.
  29. Cushing CM, Petronek MS, Bodeker KL, Vollstedt S, Brown HA, Opat E, Hollenbeck NJ, Shanks T, Berg DJ, Smith BJ, Smith MC, Monga V, Furqan M, Howard MA, Greenlee JD, Mapuskar KA, St-Aubin J, Flynn RT, Cullen JJ, Buettner GR, Spitz DR, Buatti JM, Allen BG, Magnotta VA. Magnetic resonance imaging (MRI) of pharmacological ascorbate-induced iron redox state as a biomarker in subjects undergoing radio-chemotherapy. Redox Biol. 2021 Jan;38:101804. doi: 10.1016/j.redox.2020.101804. Epub 2020 Nov 19. PMID: 33260088; PMCID: PMC7708874.
  30. Petronek MS, Steinbach EJ, Kalen AL, Builta ZJ, Callaghan CM, Hyer DE, Spitz DR, Flynn RT, Buatti JM, Magnotta VA, Zepeda-Orozco D, St-Aubin JJ, Allen BG. Assessment of Gadobutrol Safety in Combination with Ionizing Radiation Using a Preclinical MRI-Guided Radiotherapy Model. Radiat Res. 2021 Mar 1;195(3):230-234. doi: 10.1667/RADE-20-00199.1. PMID: 33347596; PMCID: PMC8011992.
  31. Ma DC, Anderson CM, Rodman SN, Buranasudja V, McCormick ML, Davis A, Loth E, Bodeker KL, Ahmann L, Parkhurst JR, Sun W, Follmer KR, Simons AL, Buatti JM, Spitz DR, Fath MA, Allen BG. Ketogenic Diet with Concurrent Chemoradiation in Head and Neck Squamous Cell Carcinoma: Preclinical and Phase 1 Trial Results. Radiat Res. 2021 Aug 1;196(2):213-224. doi: 10.1667/RADE-20-00150.1. PMID: 34087943; PMCID: PMC8440425.
  32. O’Leary BR, Carroll RS, Steers GJ, Hrabe J, Domann FE, Cullen JJ. Impact of EcSOD Perturbations in Cancer Progression. Antioxidants (Basel). 2021 Jul 29;10(8):1219. doi: 10.3390/antiox10081219. PMID: 34439467; PMCID: PMC8388922.
  33. Du J, Carroll RS, Steers GJ, Wagner BA, O’Leary BR, Jensen CS, Buettner GR, Cullen JJ. Catalase Modulates the Radio-Sensitization of Pancreatic Cancer Cells by Pharmacological Ascorbate. Antioxidants (Basel). 2021 Apr 16;10(4):614. doi: 10.3390/antiox10040614. PMID: 33923601; PMCID: PMC8073689.
  34. Petronek MS, Stolwijk JM, Murray SD, Steinbach EJ, Zakharia Y, Buettner GR, Spitz DR, Allen BG. Utilization of redox modulating small molecules that selectively act as pro-oxidants in cancer cells to open a therapeutic window for improving cancer therapy. Redox Biol. 2021 Jun;42:101864. doi: 10.1016/j.redox.2021.101864. Epub 2021 Jan 16. PMID: 33485837; PMCID: PMC8113052.
  35. Mehdi Z, Petronek MS, Stolwijk JM, Mapuskar KA, Kalen AL, Buettner GR, Cullen JJ, Spitz DR, Buatti JM, Allen BG. Utilization of Pharmacological Ascorbate to Enhance Hydrogen Peroxide-Mediated Radiosensitivity in Cancer Therapy. Int J Mol Sci. 2021 Oct 8;22(19):10880. doi: 10.3390/ijms221910880. PMID: 34639220; PMCID: PMC8509557.
  36. Mapuskar KA, Steinbach EJ, Zaher A, Riley DP, Beardsley RA, Keene JL, Holmlund JT, Anderson CM, Zepeda-Orozco D, Buatti JM, Spitz DR, Allen BG. Mitochondrial Superoxide Dismutase in Cisplatin-Induced Kidney Injury. Antioxidants (Basel). 2021 Aug 24;10(9):1329. doi: 10.3390/antiox10091329. PMID: 34572961; PMCID: PMC8469643.
  37. Steers GJ, Carroll RS, O’Leary BR, Cullen JJ. Epigenetic effects of pharmacologic ascorbate. Oncotarget. 2021 Apr 27;12(9):876-877. doi: 10.18632/oncotarget.27911. PMID: 33953841; PMCID: PMC8092345.
  38. Petronek MS, Spitz DR, Allen BG. Iron-Sulfur Cluster Biogenesis as a Critical Target in Cancer. Antioxidants (Basel). 2021 Sep 14;10(9):1458. doi: 10.3390/antiox10091458. PMID: 34573089; PMCID: PMC8465902.
  39. Petronek MS, St-Aubin JJ, Lee CY, Spitz DR, Gillan EG, Allen BG, Magnotta VA. Quantum chemical insight into the effects of the local electron environment on T2*-based MRI. Sci Rep. 2021 Oct 21;11(1):20817. doi: 10.1038/s41598-021-00305-7. PMID: 34675308; PMCID: PMC8531323.
  40. Steers GJ, Chen GY, O’Leary BR, Du J, Van Beek H, Cullen JJ. Auranofin and Pharmacologic Ascorbate as Radiomodulators in the Treatment of Pancreatic Cancer. Antioxidants (Basel). 2022 May 14;11(5):971. doi: 10.3390/antiox11050971. PMID: 35624835; PMCID: PMC9137675.
  41. O’Leary BR, Ruppenkamp EK, Steers GJ, Du J, Carroll RS, Wagner BA, Buettner GR, Cullen JJ. Pharmacological Ascorbate Enhances Chemotherapies in Pancreatic Ductal Adenocarcinoma. Pancreas. 2022 Jul 1;51(6):684-693. doi: 10.1097/MPA.0000000000002086. Epub 2022 Sep 13. PMID: 36099493; PMCID: PMC9547864.
  42. Furqan M, Abu-Hejleh T, Stephens LM, Hartwig SM, Mott SL, Pulliam CF, Petronek M, Henrich JB, Fath MA, Houtman JC, Varga SM, Bodeker KL, Bossler AD, Bellizzi AM, Zhang J, Monga V, Mani H, Ivanovic M, Smith BJ, Byrne MM, Zeitler W, Wagner BA, Buettner GR, Cullen JJ, Buatti JM, Spitz DR, Allen BG. Pharmacological ascorbate improves the response to platinum-based chemotherapy in advanced stage non-small cell lung cancer. Redox Biol. 2022 Jul;53:102318. doi: 10.1016/j.redox.2022.102318. Epub 2022 Apr 20. PMID: 35525024; PMCID: PMC9079696.
  43. Du J, Pope AN, O’Leary BR, Wagner BA, Goswami PC, Buettner GR, Cullen JJ. The role of mitochondria in pharmacological ascorbate-induced toxicity. Sci Rep. 2022 Dec 29;12(1):22521. doi: 10.1038/s41598-022-27185-9. PMID: 36581766; PMCID: PMC9800562.
  44. Du J, Pope AN, O’Leary BR, Wagner BA, Goswami PC, Buettner GR, Cullen JJ. The role of mitochondria in pharmacological ascorbate-induced toxicity. Sci Rep. 2022 Dec 29;12(1):22521. doi: 10.1038/s41598-022-27185-9. PMID: 36581766; PMCID: PMC9800562.
  45. Wagner BA, Buettner GR. Stability of aqueous solutions of ascorbate for basic research and for intravenous administration. Adv Redox Res. 2023 Dec;9:100077. doi: 10.1016/j.arres.2023.100077. Epub 2023 Jul 16. PMID: 37808406; PMCID: PMC10552410.
  46. Mapuskar KA, Pulliam CF, Zepeda-Orozco D, Griffin BR, Furqan M, Spitz DR, Allen BG. Redox Regulation of Nrf2 in Cisplatin-Induced Kidney Injury. Antioxidants (Basel). 2023 Sep 6;12(9):1728. doi: 10.3390/antiox12091728. PMID: 37760031; PMCID: PMC10525889.
  47. Steers GJ, O’Leary BR, Du J, Wagner BA, Carroll RS, Domann FE, Goswami PC, Buettner GR, Cullen JJ. Pharmacologic Ascorbate and DNMT Inhibitors Increase DUOX Expression and Peroxide-Mediated Toxicity in Pancreatic Cancer. Antioxidants (Basel). 2023 Aug 29;12(9):1683. doi: 10.3390/antiox12091683. PMID: 37759986; PMCID: PMC10525653.
  48. O’Leary BR, Kalen AL, Pope AN, Goswami PC, Cullen JJ. Hydrogen Peroxide Mediates Pharmacological Ascorbate Induced Radio-Sensitization of Pancreatic Cancer Cells by Enhancing G2-accumulation and Reducing Cyclin B1 Protein Levels. Radiat Res. 2023 Nov 1;200(5):444-455. doi: 10.1667/RADE-22-00182.1. PMID: 37758045; PMCID: PMC10699322.
  49. Petronek MS, Teferi N, Caster JM, Stolwijk JM, Zaher A, Buatti JM, Hasan D, Wafa EI, Salem AK, Gillan EG, St-Aubin JJ, Buettner GR, Spitz DR, Magnotta VA, Allen BG. Magnetite nanoparticles as a kinetically favorable source of iron to enhance GBM response to chemoradiosensitization with pharmacological ascorbate. Redox Biol. 2023 Jun;62:102651. doi: 10.1016/j.redox.2023.102651. Epub 2023 Mar 7. PMID: 36924683; PMCID: PMC10025281.
  50. Carroll RS, Du J, O’Leary BR, Steers G, Goswami PC, Buettner GR, Cullen JJ. Pharmacological ascorbate induces sustained mitochondrial dysfunction. Free Radic Biol Med. 2023 Aug 1;204:108-117. doi: 10.1016/j.freeradbiomed.2023.04.023. Epub 2023 May 1. PMID: 37137343; PMCID: PMC10375417.
  51. Singhania M, Zaher A, Pulliam CF, Bayanbold K, Searby CC, Schoenfeld JD, Mapuskar KA, Fath MA, Allen BG, Spitz DR, Petronek MS. Quantitative MRI Evaluation of Ferritin Overexpression in Non-Small-Cell Lung Cancer. Int J Mol Sci. 2024 Feb 18;25(4):2398. doi: 10.3390/ijms25042398. PMID: 38397073; PMCID: PMC10889593.
  52. Petronek MS, Monga V, Bodeker KL, Kwofie M, Lee CY, Mapuskar KA, Stolwijk JM, Zaher A, Wagner BA, Smith MC, Vollstedt S, Brown H, Chandler ML, Lorack AC, Wulfekuhle JS, Sarkaria JN, Flynn RT, Greenlee JDW, Howard MA, Smith BJ, Jones KA, Buettner GR, Cullen JJ, St-Aubin J, Buatti JM, Magnotta VA, Spitz DR, Allen BG. Magnetic Resonance Imaging of Iron Metabolism with T2* Mapping Predicts an Enhanced Clinical Response to Pharmacologic Ascorbate in Patients with GBM. Clin Cancer Res. 2024 Jan 17;30(2):283-293. doi: 10.1158/1078-0432.CCR-22-3952. PMID: 37773633; PMCID: PMC10841843.
  53. Chen GY, O’Leary BR, Du J, Carroll RS, Steers GJ, Buettner GR, Cullen JJ. Pharmacologic Ascorbate Radiosensitizes Pancreatic Cancer but Radioprotects Normal Tissue: The Role of Oxidative Stress-Induced Lipid Peroxidation. Antioxidants (Basel). 2024 Mar 18;13(3):361. doi: 10.3390/antiox13030361. PMID: 38539894; PMCID: PMC10967795.
  54. Bodeker KL, Smith BJ, Berg DJ, Chandrasekharan C, Sharif S, Fei N, Vollstedt S, Brown H, Chandler M, Lorack A, McMichael S, Wulfekuhle J, Wagner BA, Buettner GR, Allen BG, Caster JM, Dion B, Kamgar M, Buatti JM, Cullen JJ. A randomized trial of pharmacological ascorbate, gemcitabine, and nab-paclitaxel for metastatic pancreatic cancer. Redox Biol. 2024 Nov;77:103375. doi: 10.1016/j.redox.2024.103375. Epub 2024 Oct 2. PMID: 39369582; PMCID: PMC11491967.